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lenti x qrt pcr titration kit  (TaKaRa)
96
TaKaRa lenti x qrt pcr titration kit
(A) Schematic representation of the transfer vector used in this study. (B) Average virus titer (viral genome copies/mL) of each LV (n = 5). (C) A total of 2.5x10 5 NK92 cells were transduced with each type of lentiviral particle using 2.32x10 9 viral genome copies. The CAR expression level of each CAR-NK92 cell group was assessed by CD19 scFv-positive cell percentage on days 3, 7, and 14 post-transduction (day 3, n = 4; days 7 and 14, n = 5). (D) Cytotoxic activity of untransduced (UTD) and CAR-NK92 cells against K562 and Nalm-6. Cells were co-cultured for 4 hours at indicated E:T ratios (n = 4). <t>(E)</t> <t>qRT-PCR</t> analysis of the relative gene expression of CAR (n = 3). Data are shown as relative CD19 scFv gene expression using human GAPDH as a reference gene with analysis by the 2-ΔΔC T algorithm. (F) Gel electrophoresis analysis of CD19 scFv and human GAPDH amplified from genomic DNA of both UTD and CAR-NK92 cells at days 7 and 14 after transduction. DNA was extracted as total cellular genomic DNA, which may also contain non-integrated residual viral genomes. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (C), and one-way ANOVA with Tukey’s multiple comparisons test (B, E). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, non-significant.
Lenti X Qrt Pcr Titration Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/titration/pmc13193545-52-10-15?v=TaKaRa
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lenti x qrt pcr titration kit - by Bioz Stars, 2026-08
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g20 compact titrator  (METTLER TOLEDO)
90
METTLER TOLEDO g20 compact titrator
(A) Schematic representation of the transfer vector used in this study. (B) Average virus titer (viral genome copies/mL) of each LV (n = 5). (C) A total of 2.5x10 5 NK92 cells were transduced with each type of lentiviral particle using 2.32x10 9 viral genome copies. The CAR expression level of each CAR-NK92 cell group was assessed by CD19 scFv-positive cell percentage on days 3, 7, and 14 post-transduction (day 3, n = 4; days 7 and 14, n = 5). (D) Cytotoxic activity of untransduced (UTD) and CAR-NK92 cells against K562 and Nalm-6. Cells were co-cultured for 4 hours at indicated E:T ratios (n = 4). <t>(E)</t> <t>qRT-PCR</t> analysis of the relative gene expression of CAR (n = 3). Data are shown as relative CD19 scFv gene expression using human GAPDH as a reference gene with analysis by the 2-ΔΔC T algorithm. (F) Gel electrophoresis analysis of CD19 scFv and human GAPDH amplified from genomic DNA of both UTD and CAR-NK92 cells at days 7 and 14 after transduction. DNA was extracted as total cellular genomic DNA, which may also contain non-integrated residual viral genomes. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (C), and one-way ANOVA with Tukey’s multiple comparisons test (B, E). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, non-significant.
G20 Compact Titrator, supplied by METTLER TOLEDO, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/titration/10__22175_slash_mmb__17650-54-26-35?v=METTLER+TOLEDO
Average 90 stars, based on 1 article reviews
g20 compact titrator - by Bioz Stars, 2026-08
90/100 stars
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virus titration kit  (Applied Biological Materials Inc)
86
Applied Biological Materials Inc virus titration kit
(A) Schematic representation of the transfer vector used in this study. (B) Average virus titer (viral genome copies/mL) of each LV (n = 5). (C) A total of 2.5x10 5 NK92 cells were transduced with each type of lentiviral particle using 2.32x10 9 viral genome copies. The CAR expression level of each CAR-NK92 cell group was assessed by CD19 scFv-positive cell percentage on days 3, 7, and 14 post-transduction (day 3, n = 4; days 7 and 14, n = 5). (D) Cytotoxic activity of untransduced (UTD) and CAR-NK92 cells against K562 and Nalm-6. Cells were co-cultured for 4 hours at indicated E:T ratios (n = 4). <t>(E)</t> <t>qRT-PCR</t> analysis of the relative gene expression of CAR (n = 3). Data are shown as relative CD19 scFv gene expression using human GAPDH as a reference gene with analysis by the 2-ΔΔC T algorithm. (F) Gel electrophoresis analysis of CD19 scFv and human GAPDH amplified from genomic DNA of both UTD and CAR-NK92 cells at days 7 and 14 after transduction. DNA was extracted as total cellular genomic DNA, which may also contain non-integrated residual viral genomes. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (C), and one-way ANOVA with Tukey’s multiple comparisons test (B, E). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, non-significant.
Virus Titration Kit, supplied by Applied Biological Materials Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/titration/pm42320477-262-13-16?v=Applied+Biological+Materials+Inc
Average 86 stars, based on 1 article reviews
virus titration kit - by Bioz Stars, 2026-08
86/100 stars
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kjeltectm 8400 titration apparatus  (Foss Analytical)
86
Foss Analytical kjeltectm 8400 titration apparatus
(A) Schematic representation of the transfer vector used in this study. (B) Average virus titer (viral genome copies/mL) of each LV (n = 5). (C) A total of 2.5x10 5 NK92 cells were transduced with each type of lentiviral particle using 2.32x10 9 viral genome copies. The CAR expression level of each CAR-NK92 cell group was assessed by CD19 scFv-positive cell percentage on days 3, 7, and 14 post-transduction (day 3, n = 4; days 7 and 14, n = 5). (D) Cytotoxic activity of untransduced (UTD) and CAR-NK92 cells against K562 and Nalm-6. Cells were co-cultured for 4 hours at indicated E:T ratios (n = 4). <t>(E)</t> <t>qRT-PCR</t> analysis of the relative gene expression of CAR (n = 3). Data are shown as relative CD19 scFv gene expression using human GAPDH as a reference gene with analysis by the 2-ΔΔC T algorithm. (F) Gel electrophoresis analysis of CD19 scFv and human GAPDH amplified from genomic DNA of both UTD and CAR-NK92 cells at days 7 and 14 after transduction. DNA was extracted as total cellular genomic DNA, which may also contain non-integrated residual viral genomes. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (C), and one-way ANOVA with Tukey’s multiple comparisons test (B, E). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, non-significant.
Kjeltectm 8400 Titration Apparatus, supplied by Foss Analytical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/titration/pm42285674-115-11-15?v=Foss+Analytical
Average 86 stars, based on 1 article reviews
kjeltectm 8400 titration apparatus - by Bioz Stars, 2026-08
86/100 stars
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potentiometric titrator  (Hanna Instruments)
86
Hanna Instruments potentiometric titrator
(A) Schematic representation of the transfer vector used in this study. (B) Average virus titer (viral genome copies/mL) of each LV (n = 5). (C) A total of 2.5x10 5 NK92 cells were transduced with each type of lentiviral particle using 2.32x10 9 viral genome copies. The CAR expression level of each CAR-NK92 cell group was assessed by CD19 scFv-positive cell percentage on days 3, 7, and 14 post-transduction (day 3, n = 4; days 7 and 14, n = 5). (D) Cytotoxic activity of untransduced (UTD) and CAR-NK92 cells against K562 and Nalm-6. Cells were co-cultured for 4 hours at indicated E:T ratios (n = 4). <t>(E)</t> <t>qRT-PCR</t> analysis of the relative gene expression of CAR (n = 3). Data are shown as relative CD19 scFv gene expression using human GAPDH as a reference gene with analysis by the 2-ΔΔC T algorithm. (F) Gel electrophoresis analysis of CD19 scFv and human GAPDH amplified from genomic DNA of both UTD and CAR-NK92 cells at days 7 and 14 after transduction. DNA was extracted as total cellular genomic DNA, which may also contain non-integrated residual viral genomes. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (C), and one-way ANOVA with Tukey’s multiple comparisons test (B, E). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, non-significant.
Potentiometric Titrator, supplied by Hanna Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/titration/pm42285489-43-11-15?v=Hanna+Instruments
Average 86 stars, based on 1 article reviews
potentiometric titrator - by Bioz Stars, 2026-08
86/100 stars
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karl fischer titrator  (Nitto Pharmaceutical Industries)
86
Nitto Pharmaceutical Industries karl fischer titrator
(A) Schematic representation of the transfer vector used in this study. (B) Average virus titer (viral genome copies/mL) of each LV (n = 5). (C) A total of 2.5x10 5 NK92 cells were transduced with each type of lentiviral particle using 2.32x10 9 viral genome copies. The CAR expression level of each CAR-NK92 cell group was assessed by CD19 scFv-positive cell percentage on days 3, 7, and 14 post-transduction (day 3, n = 4; days 7 and 14, n = 5). (D) Cytotoxic activity of untransduced (UTD) and CAR-NK92 cells against K562 and Nalm-6. Cells were co-cultured for 4 hours at indicated E:T ratios (n = 4). <t>(E)</t> <t>qRT-PCR</t> analysis of the relative gene expression of CAR (n = 3). Data are shown as relative CD19 scFv gene expression using human GAPDH as a reference gene with analysis by the 2-ΔΔC T algorithm. (F) Gel electrophoresis analysis of CD19 scFv and human GAPDH amplified from genomic DNA of both UTD and CAR-NK92 cells at days 7 and 14 after transduction. DNA was extracted as total cellular genomic DNA, which may also contain non-integrated residual viral genomes. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (C), and one-way ANOVA with Tukey’s multiple comparisons test (B, E). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, non-significant.
Karl Fischer Titrator, supplied by Nitto Pharmaceutical Industries, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/titration/pm42264055-95-10-14?v=Nitto+Pharmaceutical+Industries
Average 86 stars, based on 1 article reviews
karl fischer titrator - by Bioz Stars, 2026-08
86/100 stars
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kjeldahl distillation titration analyzer  (Foss Analytical)
86
Foss Analytical kjeldahl distillation titration analyzer
(A) Schematic representation of the transfer vector used in this study. (B) Average virus titer (viral genome copies/mL) of each LV (n = 5). (C) A total of 2.5x10 5 NK92 cells were transduced with each type of lentiviral particle using 2.32x10 9 viral genome copies. The CAR expression level of each CAR-NK92 cell group was assessed by CD19 scFv-positive cell percentage on days 3, 7, and 14 post-transduction (day 3, n = 4; days 7 and 14, n = 5). (D) Cytotoxic activity of untransduced (UTD) and CAR-NK92 cells against K562 and Nalm-6. Cells were co-cultured for 4 hours at indicated E:T ratios (n = 4). <t>(E)</t> <t>qRT-PCR</t> analysis of the relative gene expression of CAR (n = 3). Data are shown as relative CD19 scFv gene expression using human GAPDH as a reference gene with analysis by the 2-ΔΔC T algorithm. (F) Gel electrophoresis analysis of CD19 scFv and human GAPDH amplified from genomic DNA of both UTD and CAR-NK92 cells at days 7 and 14 after transduction. DNA was extracted as total cellular genomic DNA, which may also contain non-integrated residual viral genomes. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (C), and one-way ANOVA with Tukey’s multiple comparisons test (B, E). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, non-significant.
Kjeldahl Distillation Titration Analyzer, supplied by Foss Analytical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/titration/pmc13053785-159-35-58?v=Foss+Analytical
Average 86 stars, based on 1 article reviews
kjeldahl distillation titration analyzer - by Bioz Stars, 2026-08
86/100 stars
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karl fischer titration  (Kyoto Scientific Instrument Association)
86
Kyoto Scientific Instrument Association karl fischer titration
(A) Schematic representation of the transfer vector used in this study. (B) Average virus titer (viral genome copies/mL) of each LV (n = 5). (C) A total of 2.5x10 5 NK92 cells were transduced with each type of lentiviral particle using 2.32x10 9 viral genome copies. The CAR expression level of each CAR-NK92 cell group was assessed by CD19 scFv-positive cell percentage on days 3, 7, and 14 post-transduction (day 3, n = 4; days 7 and 14, n = 5). (D) Cytotoxic activity of untransduced (UTD) and CAR-NK92 cells against K562 and Nalm-6. Cells were co-cultured for 4 hours at indicated E:T ratios (n = 4). <t>(E)</t> <t>qRT-PCR</t> analysis of the relative gene expression of CAR (n = 3). Data are shown as relative CD19 scFv gene expression using human GAPDH as a reference gene with analysis by the 2-ΔΔC T algorithm. (F) Gel electrophoresis analysis of CD19 scFv and human GAPDH amplified from genomic DNA of both UTD and CAR-NK92 cells at days 7 and 14 after transduction. DNA was extracted as total cellular genomic DNA, which may also contain non-integrated residual viral genomes. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (C), and one-way ANOVA with Tukey’s multiple comparisons test (B, E). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, non-significant.
Karl Fischer Titration, supplied by Kyoto Scientific Instrument Association, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/titration/pmc13177167-180-6-9?v=Kyoto+Scientific+Instrument+Association
Average 86 stars, based on 1 article reviews
karl fischer titration - by Bioz Stars, 2026-08
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titratable acidity  (Hanna Instruments)
86
Hanna Instruments titratable acidity

Titratable Acidity, supplied by Hanna Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/titration/bio_rxiv__64898__2026__05__09__723973-44-22-16?v=Hanna+Instruments
Average 86 stars, based on 1 article reviews
titratable acidity - by Bioz Stars, 2026-08
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Image Search Results


(A) Schematic representation of the transfer vector used in this study. (B) Average virus titer (viral genome copies/mL) of each LV (n = 5). (C) A total of 2.5x10 5 NK92 cells were transduced with each type of lentiviral particle using 2.32x10 9 viral genome copies. The CAR expression level of each CAR-NK92 cell group was assessed by CD19 scFv-positive cell percentage on days 3, 7, and 14 post-transduction (day 3, n = 4; days 7 and 14, n = 5). (D) Cytotoxic activity of untransduced (UTD) and CAR-NK92 cells against K562 and Nalm-6. Cells were co-cultured for 4 hours at indicated E:T ratios (n = 4). (E) qRT-PCR analysis of the relative gene expression of CAR (n = 3). Data are shown as relative CD19 scFv gene expression using human GAPDH as a reference gene with analysis by the 2-ΔΔC T algorithm. (F) Gel electrophoresis analysis of CD19 scFv and human GAPDH amplified from genomic DNA of both UTD and CAR-NK92 cells at days 7 and 14 after transduction. DNA was extracted as total cellular genomic DNA, which may also contain non-integrated residual viral genomes. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (C), and one-way ANOVA with Tukey’s multiple comparisons test (B, E). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, non-significant.

Journal: PLOS One

Article Title: BaEV-pseudotyped lentiviral vectors enable stable CAR expression and cytotoxic function in NK cells

doi: 10.1371/journal.pone.0348674

Figure Lengend Snippet: (A) Schematic representation of the transfer vector used in this study. (B) Average virus titer (viral genome copies/mL) of each LV (n = 5). (C) A total of 2.5x10 5 NK92 cells were transduced with each type of lentiviral particle using 2.32x10 9 viral genome copies. The CAR expression level of each CAR-NK92 cell group was assessed by CD19 scFv-positive cell percentage on days 3, 7, and 14 post-transduction (day 3, n = 4; days 7 and 14, n = 5). (D) Cytotoxic activity of untransduced (UTD) and CAR-NK92 cells against K562 and Nalm-6. Cells were co-cultured for 4 hours at indicated E:T ratios (n = 4). (E) qRT-PCR analysis of the relative gene expression of CAR (n = 3). Data are shown as relative CD19 scFv gene expression using human GAPDH as a reference gene with analysis by the 2-ΔΔC T algorithm. (F) Gel electrophoresis analysis of CD19 scFv and human GAPDH amplified from genomic DNA of both UTD and CAR-NK92 cells at days 7 and 14 after transduction. DNA was extracted as total cellular genomic DNA, which may also contain non-integrated residual viral genomes. Data are presented as mean ± standard deviation (SD). Statistical analysis was performed using two-way ANOVA with Tukey’s multiple comparisons test (C), and one-way ANOVA with Tukey’s multiple comparisons test (B, E). *, p < 0.05; **, p < 0.01; ****, p < 0.0001; ns, non-significant.

Article Snippet: Subsequently, the number of viral copies was quantified using the Lenti-X qRT-PCR Titration Kit (631235, Takara Bio Inc.), and the titer of virus stock was measured by multiplying the quantified number by the fold dilution of the stock.

Techniques: Plasmid Preparation, Virus, Transduction, Expressing, Activity Assay, Cell Culture, Quantitative RT-PCR, Gene Expression, Nucleic Acid Electrophoresis, Amplification, Standard Deviation

Journal: bioRxiv

Article Title: A2 and A1B in vitro milk digests: effects on in vitro leaky gut model and adipose cells

doi: 10.64898/2026.05.09.723973

Figure Lengend Snippet:

Article Snippet: On each milk pool, after 24 h from milking, pH was measured with a pH-meter (HI98191, Hanna Instruments, Villafranca Padovana, Italy), whereas titratable acidity was measured with 0.25 mol/L NaOH according to the Soxhlet-Henkel method.

Techniques: